Besides physiological processes, these helper proteins also fulfil crucial functions under unfavorable environmental conditions, such as temperature or osmotic stresses, but also in disease conditions and in response to a pathogen attack. Molecular chaperones are a diverse group of proteins that play critical roles in assisting the folding and assembly of nascent protein chains, refolding proteins, assisting protein translocation through membranes, and often facilitating protein degradation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its Supporting Information files.įunding: This work was supported by the Odysseus grant G.0029.12 from the Fonds Wetenschappelijk Onderzoek (FWO, ) to PT, and DK is supported by a FWO postdoctoral fellowship 1.2.734.13. Received: Accepted: AugPublished: August 26, 2016Ĭopyright: © 2016 Hristozova et al. Brodsky, University of Pittsburgh, UNITED STATES The use of this method may lead to a considerable increase in the number of experimentally verified proteins with such functions, and may also allow the dissection of their molecular mechanism for a better understanding of their function.Ĭitation: Hristozova N, Tompa P, Kovacs D (2016) A Novel Method for Assessing the Chaperone Activity of Proteins. The current state of the art does not allow the in vitro measurements of chaperone activity in a highly parallel manner with high accuracy or high reproducibility, thus we believe that the method we report will be of significant benefit in this direction. Here we propose a novel technique to test and measure the capability for protective activity of known and putative chaperones in a semi-high throughput manner on a plate reader. Their molecular functions range from stabilizing stress-susceptible molecules and membranes to assisting the refolding of stress-damaged proteins, thereby acting as protective barriers against cellular damage. Among stress protein families–molecules expressed during adverse conditions, infection, and diseases–chaperones are highly abundant. Depending on their specific function, molecular chaperones are involved in a plethora of cellular processes by playing key roles in nascent protein chain folding, transport and quality control. Protein chaperones are molecular machines which function both during homeostasis and stress conditions in all living organisms.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |